Human Gastric Adenocarcinoma Cell Line Essay -- Chemistry, Cell

Introduction There are many different cell lines that have been used in order to examine genotoxicity exposure of agents such as human gastric adenocarcinoma cell line - TMK1, human colon cancer - COL2, particularly human B lymphoblastoid cell line - TK6 is more often used. TK6 is a cell line heterozygous at the thymidine kinase locus from a patient with chronic myelogenous leukemia (CML) in T cell lineage blast crisis which included Philadelphia chromosome t(9;22)(q34;q11), an abnormality in chromosome 6 (a deletion of the long arm), and chromosome 7, for example, del(6)(q21); ins(1;-)(q21;-); del(1)(q21;q32); dic(7)(:p13ïÆ'   cenïÆ'   q32:11.2ïÆ'   cenïÆ'   pter) (Watanabe et al., 1995; Tomita et al., 1998). These cells are relatively stable p53-normal immortal, have low mutation frequencies in gene and chromosome, and are proficient in mismatch repair (Tomita-Mitchel et al., 2000), from these properties TK6 cell line is applied in this study for assessing toxicity of anti-cancer agents. Polyynes containing triple bonds which are presented in many natural sources, have been considered in medicine with a variety of biological activities such as antifungal, anti-inflammatory, antibacterial, antimicrobial, antiHIV, and anticancer (Dembitsky et al., 2006). Previously, there were many researches about the diyne-structure-related-capability of polyyne, especially ene-diyne, these investigations suggested that enediyne compounds include two triple bonds (diyne) seperated by a double bond (ene) can cleavage DNA via cross linking to several positions, interacting with minor groove or abstracting hydrogen atoms as well as arrest cell cycle, inhibit proteins required for initiation replication stage (Chin et al., 1988; Sugiura et al., 1989; Walker et a... ... nuclei, and should be separated with clear nuclear boundary. Timescale involved Along with the proposal, the RAGS system will be completed and approved in week 18. After that, the first step is synthesis of two chemicals in 4 weeks from 16th January to 13th February 2012. Once the chemicals are produced, TK6 cells will be treated in control medium and in medium containing these compounds, separately, and measure LD50 ¬ of TK6 cell line, statistic analysis LOEC, NOEC and thereby comparing cytotoxicity of these compounds. This process will take around 6 weeks from 13th February to 23rd March 2012. Later on, micronucleus assay will be applied to study genotoxicity on TK6 cells via clastogenic behaviour for 7 weeks and finish laboratory practical on 1st June 2012. Finally, writing up and project draft will be completed within 4 weeks.

Integrated DNS Servers

I am working at two branch offices and have been tasked with where to place the Active- Directory Integrated DNS Servers and what type to use. One of the branch offices is very small and (5 users) and has a very slow network connectivity. Do I need a DNS Server and, if so, which type of zone should it hosts? The second branch office is much larger (about 30 users) and has better network connectivity. Does this office need a DNS Server and, if so what type of zone would you recommend?Response:Dear Junior Admin; I really appreciate the opportunity to assist you in regards to implementing Active Directory & DNS Servers in your â€Å"Windows† environment. Let me start by saying that without DNS your network will more than likely not function because clients will not be able to resolve names to (IP) addresses, also DNS enables network devices such as printers and computers to communicate on the internet or locate one another within the organizations local network. Based on the give n scenario, you have made an excellent choice of configuring the â€Å"Active Directory Integrated Zones† because Active Directory has the following benefits: Fault Tolerance – Redundant copy of DNS zone information can be stored on multiple servers. Security – DACL can be modified by specified user groups.Zones are Multimaster – zones can be updated in more than one location. Efficient Replication – Zone transfers are replaced by more efficient Active Directory replication. Maintain use of secondary zones – if needed.Note: Since Active Directory-Integrated Zones follow a multimaster update model which means all zones contain a read/write copy of the zone and can make changes to the zone information, â€Å"primary and secondary distinctions are not necessary.† The smaller Branch office with (5) users will require will house what would normally be your Secondary Server, utilizing the Active Directory with Integrated Zones will compensa te for the slow network because it will avoid forcing queries across a slow wide area network link. The larger Branch office with (30) users certainly requires the utilization of DNS & Active Directory Integrated Zones† and will house what would

Heroes in the Epic Stories of Odyssey, Aeneid, and Antigone

Throughout time, heroic tales have been a source of entertainment. For many, they offer hope and inspiration. Heroic tales often involve a battle, not necessarily a war, but a battle of will or good versus evil. The protagonists in Odyssey, Aeneid, and Antigone are considered heroes. When faced with options for which way their life will go, these characters choose the more difficult path. They never give up on what they believe in. They continue their path because of there courageous since of duty and destiny. While cultural changes dictate what is considered heroic, bravery and self-sacrifice remain constant throughout time. To begin, Homer’s Odyssey tells the epic story of the Greek hero Odysseus and his struggle to return home. Odysseus was away from home for twenty years; he left behind a loving wife and a newborn son. He sacrificed his home and family to wage war against the Trojans. The epic tale of The Odyssey begins at the end of a ten-year battle in Troy; Odysseus is instrumental in winning this battle. Odysseus is clever and quite cunning; he often uses this throughout his adventures to escape danger. Odysseus devises a plan for the Trojan horse that leads to the downfall of Troy. This plan alone would have been enough to gain â€Å"hero† status in his time. In the Greek culture, warriors often achieve immortal fame through heroic deeds in battle. Once the battle is over, Odysseus’ focus changes to his journey home. During this journey, he faces with many obstacles.Show MoreRelatedGreek Mythology8088 Words   |  33 Pagesabout  the  exploits  of  gods   and  heroes  and  their  relations  with  ordinary  mortals.   The  ancient  Greeks  worshiped  many  gods  within  a  culture  that  tolerated  diversity.  Unlike  other  belief   systems,  Greek  culture  recognized  no  single  truth  or  code  and  produced  no  sacred,  written  text  likeà ‚   the  Bible  or  the  Qur’an.  Stories  about  the  origins  and  actions  of  Greek  divinities  varied  widely,   depending,  for  example,  on  whether  the  tale  appeared  in  a  comedy,  tragedy,  or  epic  poem.  Greek   mythology  was  like  a  complex  and  rich  language